Evolution of carbapenem resistance in klebsiella pneumoniae and escherichia coli carrying blaNDM−1 gene: imipenem exposure results in sustained resistance memory of strains in vitro

Table 1 The list of primers used for the amplification of β-lactamase genes

Gene	Primer sequence (5^'→3^')	Gene length (bp)	Tm (℃)
bla _SHV ^a	F:ATGCGTTATATTCGCCTGTG	843	55
	R:TTAGCGTTGCCAGTGCTC
bla _TEM−1 ^b	F:AGTATTCAACATTTTCGTGT	860	49
	R:TAATCAGTGAGGCACCTATCTC
bla _CTX−M−9	F:CGTATTGGGAGTTTGAGATG	522	50
	R:GGTATTCAGCGTAGGTTC
bla _IMP	F:GCGTTTATGTTCATACTTCGTT	631	56
	R:GCTTCTAAATTTGCGTCACC
bla _NDM−1	F:ATGGAATTGCCCAATATTATGCAC	813	55
	R:TCAGCGCAGCTTGTCGGCCATGCG
bla _KPC	F:CGTTCTTGTCTCTCATGGCC	796	52
	R:CCTCGCTGTGCTTGTCATCC
bla _OXA	F:CTGGAATGAGAATAAGCAGCAA	545	57
	R:GTTCAACCCAACCGACCC
bla _GES	F:ATGCGCTTCATTCACGCAC	863	56
	R:CTATTTGTCCGTGCTCAGGA
bla _DHA	F:GTTGCCGTCTCCGTAAAG	925	50
	R:GAATCACAATCGCCACCT

ISSN: 1476-0711