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Table 1 Primer and probe sequences for the in-house RT-PCR assay

From: Comparison of in-house and commercial real time-PCR based carbapenemase gene detection methods in Enterobacteriaceae and non-fermenting gram-negative bacterial isolates

Gene

Primer pairs

Product size, bp

Probe

bla KPC

for-GCGATACCACGTTCCGTCTG

rev-CGGTCGTGTTTCCCTTTAGC

183

6FAM-AGCGGCAGCAGTTTGTTGATTG–BBQ

bla NDM

for-TTTGGCGATCTGGTTTTCCG

rev-ATCAAACCGTTGGAAGCGAC

101

LC610-AGACATTCGGTGCGAGCTGGC–BBQ

bla OXA-23-like

for-CGCGACGTATCGGTCTTGAT

rev-CGCGACGTATCGGTCTTGAT

162

HEX-ACGTATTGGTTTCGGTAATGCTGA–BBQ

bla OXA-48-like

for-GGCACGTATGAGCAAGATGC

rev-GTTTGACAATACGCTGGCTGC

182

a

bla VIM

for-CCGAGTGGTGAGTATCCGAC

rev-GAATGCGTGGGAATCTCGTTC

337

Cy5-CGCTGTATCAATCAAAAGCAACTCATCA–BBQ

  1. aThe melting-curve analysis for the OXA gene variants was performed with the OXA-48 primer. For the multiplex RT-PCR analysis a Light Mix Modular from TIB MOLBIOL Syntheselabor GmbH Berlin was used for OXA-48